ProtocolPDF · 1.2 MB
Standard Operating Procedure: WHO Tube Bioassays (Adult Vectors)
Step-by-step methodology for evaluating Anopheles vector susceptibility to diagnostic WHO insecticides (exposure time, KD tracking, and 24h mortality).
Open Science & Tooling
Academic resources,SOPs, glossary & knowledge base
An open-access scientific knowledge hub providing standard operating protocols, a bilingual medical glossary, and an intelligent research assistant.
PDFWHO SOPs & Protocols
FR/ENMedical Glossary
AIVirtual Assistant
07 — Resources & Training Hub
Academic resources center& laboratory protocols
Standard operating procedures (WHO/GLP SOPs), methodological guidelines, and training materials for students, researchers, and health technicians.
GuidePDF · 2.8 MB
Field Guide for Larval Sampling & Ecological Habitat Characterization
Identification keys for temporary and permanent breeding sites of Anopheles gambiae s.l. with field ecological survey sheets.
CoursePDF · 4.5 MB
Lecture Slides: Molecular Mechanisms of Insecticide Resistance (kdr, ace-1 & P450)
Postgraduate Master-level slides in Medical Entomology detailing target-site gene mutations and metabolic enzymatic detoxification pathways.
Technical SheetPDF · 950 KB
Standard Insectary Operations & Anopheles Colony Rearing Manual
Optimal climate parameters (27°C ± 2), relative humidity (80% ± 10), membrane blood feeding, and photoperiod cycles for reference susceptible colonies.
09 — Terminology & Scientific Glossary
Bilingual glossary ofmedical entomology & public health
Rigorous definitions and operational concepts of vector control, insecticide resistance, and malaria epidemiology.
EpidemiologyTIE / EIR
Entomological Inoculation Rate
🇫🇷 Taux d'Inoculation EntomologiqueThe number of infectious mosquito bites received by an individual over a specified time period (usually per year). It is the gold-standard metric for malaria transmission intensity.
Version française :
Nombre de piqûres de moustiques infectés par le Plasmodium reçues par une personne pendant une période donnée (généralement par an ou par nuit). C'est l'indicateur direct de référence de la pression de transmission du paludisme.
BioassaysKDT50 / KDT95
Knockdown Time
🇫🇷 Temps de KnockdownThe time required to immobilize 50% or 95% of exposed mosquitoes during a standard bioassay. A delayed knockdown time is an early indicator of knockdown resistance (kdr).
Version française :
Temps requis pour paralyser ou assommer 50% ou 95% des moustiques exposés à un insecticide lors d'un test diagnostique. Un allongement significatif du KDT est le premier signe de résistance aux pyréthroïdes.
ResistancePBO
Piperonyl Butoxide
🇫🇷 Butoxyde de PipéronyleA synergistic chemical agent that inhibits insect cytochrome P450 monooxygenases, thereby restoring pyrethroid toxicity against metabolically resistant mosquito strains.
Version française :
Composé chimique synergiste qui inhibe l'activité enzymatique des Cytochromes P450 chez le moustique, restaurant ainsi l'efficacité léthale des pyréthroïdes face aux populations à résistance métabolique.
Entomology
Anthropophily & Endophagy
🇫🇷 Anthropophilie & EndophagieAnthropophily is the preference of vectors to feed on human blood over animal hosts. Endophagy describes the habit of biting indoors rather than outdoors (exophagy).
Version française :
L'anthropophilie désigne la préférence trophique du moustique pour le sang humain plutôt qu'animal. L'endophagie désigne le comportement de piqûre à l'intérieur des habitations humaines (par opposition à l'exophagie).
Resistancekdr (L1014F / L1014S)
Knockdown Resistance (kdr)
🇫🇷 Résistance Knockdown (kdr)A target-site point mutation in the voltage-gated sodium channel gene reducing pyrethroid and DDT binding affinity. The two principal African alleles are L1014F and L1014S.
Version française :
Mutation ponctuelle sur le gène du canal sodium voltage-dépendant (Vgsc), réduisant l'affinité de liaison des pyréthroïdes et du DDT. Deux allèles majeurs prédominent en Afrique : L1014F (Ouest) et L1014S (Est).
Bioassays
Experimental Huts (Phase II)
🇫🇷 Cases Expérimentales (Phase II)A standardized semi-field facility designed to mimic human dwellings to evaluate vector deterrence, blood-feeding inhibition, and mortality induced by vector control tools.
Version française :
Dispositif de terrain semi-naturel standardisé par l'OMS simulant des habitations réelles. Permet d'évaluer la dissuasion, l'effet répulsif, l'inhibition de gorgement et la mortalité des moustiquaires ou des pulvérisations.
Resistance
Target-Site vs Metabolic Resistance
🇫🇷 Résistance de Cible vs MétaboliqueTarget-site resistance involves structural alterations in the insecticide receptor, while metabolic resistance is driven by enzymatic over-expression (P450s, GSTs, esterases).
Version française :
La résistance de cible résulte d'une mutation structurelle du récepteur (ex: kdr, ace-1), tandis que la résistance métabolique résulte de la surproduction d'enzymes détoxifiantes (Cytochromes P450, GST, Estérases).
EpidemiologyIS / SR
Sporozoite Rate
🇫🇷 Indice SporozoïtiqueThe proportion of female anophelines carrying Plasmodium sporozoites in their salivary glands, typically detected via circumsporozoite ELISA (CSP-ELISA) or PCR.
Version française :
Proportion d'anophèles femelles dont les glandes salivaires contiennent des sporozoïtes de Plasmodium falciparum, généralement déterminée par test ELISA-CSP ou PCR.
11 — Testimonials & Endorsements
Peer recognition& partner endorsements
Endorsements and testimonials from research directors, institutional partners, health agencies, and supervised early-career researchers.
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« Dr. Ahogni embodies the highest standard of West African entomological research. His rigor in conducting bioassays and his deep mastery of molecular resistance mechanisms have been decisive in shaping national vector control strategies. »
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« Working with Idelphonse on multi-country LLIN evaluation trials has been an exceptional experience. His deep operational insights in experimental huts and commitment to data integrity set a benchmark for public health entomology. »
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« Dr. Ahogni's technical expertise enabled the NMCP to seamlessly transition to next-generation dual-active and PBO nets backed by solid field evidence. »
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« A passionate scientific mentor committed to African youth. His training masterclasses on larval sampling and WHO bioassay interpretation inspire an entire generation of medical entomologists. »
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